Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Duffy antigen/chemokine receptor (DARC) Standard Curve.)
Mouse Duffy Antigen/Chemokine Receptor (DARC) ELISA Kit | DARC elisa kit
Mouse Duffy Antigen/Chemokine Receptor (DARC) ELISA Kit
Gene Names
Ackr1; FY; Dfy; GPD; Darc; CCBP1; CD234; ESTM35; AA162249
Reactivity
Mouse
Synonyms
Duffy Antigen/Chemokine Receptor (DARC); Mouse Duffy Antigen/Chemokine Receptor (DARC) ELISA Kit; DARC; CTA-134P22.3; CCBP1; CD234; Dfy; FY; GPD; GpFy; WBCQ1; Duffy blood group antigen; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294; glycoprotein D; DARC elisa kit
Reactivity
Mouse
Specificity
Mouse Duffy antigen/chemokine receptor (DARC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DARC. No significant cross-reactivity or interference between Mouse DARC and analogues was observed.
Sequence Length
334
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Duffy antigen/chemokine receptor (DARC) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Duffy antigen/chemokine receptor (DARC) Standard Curve.)
Related Product Information for DARC elisa kit
Description: This Mouse Duffy antigen/chemokine receptor (DARC) ELISA Kit employs a two-site sandwich ELISA to quantitate DARC in samples. An antibody specific for DARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DARC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by atypical chemokine receptor 1 (ACKR1)/ (Duffy antigen/chemokine receptor, DARC) is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene.
Background: The protein encoded by atypical chemokine receptor 1 (ACKR1)/ (Duffy antigen/chemokine receptor, DARC) is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
atypical chemokine receptor 1
NCBI Official Synonym Full Names
atypical chemokine receptor 1 (Duffy blood group)
NCBI Official Symbol
Ackr1
NCBI Official Synonym Symbols
FY; Dfy; GPD; Darc; CCBP1; CD234; ESTM35; AA162249
NCBI Protein Information
atypical chemokine receptor 1
UniProt Protein Name
Atypical chemokine receptor 1
UniProt Gene Name
Ackr1
UniProt Synonym Gene Names
Darc; Dfy; Fy
UniProt Entry Name
ACKR1_MOUSE
Research Articles on DARC
1. Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 are involved in neutrophil emigration.
Similar Products
Product Notes
The Mouse DARC ackr1 (Catalog #AAA9717942) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717942 ELISA Kit recognizes Mouse DARC. It is sometimes possible for the material contained within the vial of "Duffy Antigen/Chemokine Receptor (DARC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
