Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA replication licensing factor MCM2 (MCM2) Standard Curve.)
Mouse DNA Replication Licensing Factor MCM2 (MCM2) ELISA Kit | MCM2 elisa kit
Mouse DNA Replication Licensing Factor MCM2 (MCM2) ELISA Kit
Gene Names
Mcm2; BM28; CDCL1; Mcmd2; AA959861; AW476101; mKIAA0030
Reactivity
Mouse
Synonyms
DNA Replication Licensing Factor MCM2 (MCM2); Mouse DNA Replication Licensing Factor MCM2 (MCM2) ELISA Kit; MCM2; BM28; CCNL1; CDCL1; D3S3194; KIAA0030; MGC10606; MITOTIN; cdc19; DNA replication licensing factor MCM2; MCM2 minichromosome maintenance deficient 2; mitotin; cell devision cycle-like 1; cyclin-like 1; m; MCM2 elisa kit
Reactivity
Mouse
Specificity
Mouse DNA replication licensing factor MCM2 (MCM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCM2. No significant cross-reactivity or interference between Mouse MCM2 and analogues was observed.
Sequence Length
904
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA replication licensing factor MCM2 (MCM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA replication licensing factor MCM2 (MCM2) Standard Curve.)
Related Product Information for MCM2 elisa kit
Description: This Mouse DNA replication licensing factor MCM2 (MCM2) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM2 in samples. An antibody specific for MCM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DNA replication licensing factor MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
Background: DNA replication licensing factor MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 2
NCBI Official Symbol
Mcm2
NCBI Official Synonym Symbols
BM28; CDCL1; Mcmd2; AA959861; AW476101; mKIAA0030
NCBI Protein Information
DNA replication licensing factor MCM2
UniProt Protein Name
DNA replication licensing factor MCM2
Protein Family
UniProt Gene Name
Mcm2
UniProt Synonym Gene Names
Bm28; Cdcl1; Kiaa0030; Mcmd2
UniProt Entry Name
MCM2_MOUSE
Uniprot Description
MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
Protein type: EC 3.6.4.12; DNA-binding
Cellular Component: nucleoplasm; microtubule cytoskeleton; MCM complex; cytoplasm; chromatin; nuclear origin of replication recognition complex; nucleus
Molecular Function: DNA helicase activity; DNA binding; hydrolase activity; histone binding; metal ion binding; nucleotide binding; DNA replication origin binding; helicase activity; ATP binding
Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA replication; cell cycle
Research Articles on MCM2
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Product Notes
The Mouse MCM2 mcm2 (Catalog #AAA9717705) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717705 ELISA Kit recognizes Mouse MCM2. It is sometimes possible for the material contained within the vial of "DNA Replication Licensing Factor MCM2 (MCM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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