Mouse Dkk3 (Dickkopf-related protein 3) ELISA Kit | Dkk3 elisa kit

Mouse Dkk3 (Dickkopf-related protein 3) ELISA Kit

Cat. Num. AAA7607267

• Gene Names: DKK3; RIG; REIC
• Reactivity: Mouse
• Synonyms: Dkk3 (Dickkopf-related protein 3); Mouse Dkk3 (Dickkopf-related protein 3) ELISA Kit; Dkk3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DKK-1. No significant cross-reactivity or interference between DKK-1 and analogues was observed.
• Sequence Length: 350

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DKK-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DKK-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Dkk3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-DKK-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DKK-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DKK-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DKK-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 66346689
• NCBI GeneID: 27122
• NCBI Accession #: NP_001018067.1
• NCBI GenBank Nucleotide #: NM_001018057.1
• UniProt Accession #: Q9UBP4; Q9ULB7; A8K1I2; D3DQW1
• Molecular Weight: 38,390 Da
• NCBI Official Full Name: dickkopf-related protein 3 isoform 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 3
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3
• UniProt Protein Name: Dickkopf-related protein 3
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Synonym Gene Names: REIC; Dickkopf-3; Dkk-3; hDkk-3

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease ().