Mouse Dihydropyrimidine Dehydrogenase (DPYD) ELISA Kit | DPYD elisa kit

Mouse Dihydropyrimidine Dehydrogenase (DPYD) ELISA Kit

Cat. Num. AAA2023888

• Gene Names: DPYD; DHP; DPD; DHPDHASE
• Reactivity: Mouse
• Synonyms: Dihydropyrimidine Dehydrogenase (DPYD); Mouse Dihydropyrimidine Dehydrogenase (DPYD) ELISA Kit; DPD; DHPDHase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPYD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydropyrimidine Dehydrogenase (DPYD).; No significant cross-reactivity or interference between Dihydropyrimidine Dehydrogenase (DPYD) and analogues was observed.
• Sequence Length: 173

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPYD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidine Dehydrogenase (DPYD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidine Dehydrogenase (DPYD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DPYD elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 693912
• NCBI GeneID: 1806
• UniProt Accession #: Q12882; Q16694; Q16761; Q32NB0; Q96HL6; Q96TH1; A2RRQ2; A2RRQ3; A8K5A2; A8MWG9; B1AN21; E9PFN1
• Molecular Weight: 18,936 Da
• NCBI Official Full Name: dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: DPYD
• NCBI Official Synonym Symbols: DHP; DPD; DHPDHASE
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• Protein Family: Dihydropyrimidine dehydrogenase
• UniProt Gene Name: DPYD
• UniProt Synonym Gene Names: DHPDHase; DPD
• UniProt Entry Name: DPYD_HUMAN

NCBI Description

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 1.3.1.2; Nucleotide Metabolism - pyrimidine; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; 4 iron, 4 sulfur cluster binding; metal ion binding; dihydroorotate dehydrogenase activity; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; uracil catabolic process; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; thymidine catabolic process; pyrimidine base catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency