Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit | DGAT1 elisa kit

Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit

Cat. Num. AAA2706640

• Gene Names: DGAT1; ARAT; DGAT; ARGP1; DIAR7
• Reactivity: Mouse
• Synonyms: Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1); Mouse Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit; ARGP1; DGAT; ARAT; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; Diglyceride acyltransferase; DGAT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1).; No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) and analogues was observed.
• Sequence Length: 488

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DGAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145864459
• NCBI GeneID: 8694
• NCBI Accession #: NP_036211.2
• NCBI GenBank Nucleotide #: NM_012079.5
• UniProt Accession #: O75907; Q96BB8; B2RWQ2; D3DWL6
• Molecular Weight: 55,278 Da
• NCBI Official Full Name: diacylglycerol O-acyltransferase 1
• NCBI Official Synonym Full Names: diacylglycerol O-acyltransferase 1
• NCBI Official Symbol: DGAT1
• NCBI Official Synonym Symbols: ARAT; DGAT; ARGP1; DIAR7
• NCBI Protein Information: diacylglycerol O-acyltransferase 1
• UniProt Protein Name: Diacylglycerol O-acyltransferase 1
• Protein Family: Diacylglycerol O-acyltransferase
• UniProt Gene Name: DGAT1
• UniProt Synonym Gene Names: AGRP1; DGAT; ARAT; Retinol O-fatty-acyltransferase

NCBI Description

This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]

Uniprot Description

DGAT1: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Protein type: Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.20; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; plasma membrane

Molecular Function: 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity; protein binding; retinol O-fatty-acyltransferase activity; transferase activity, transferring acyl groups

Biological Process: diacylglycerol metabolic process; fatty acid homeostasis; lipid storage; neutrophil degranulation; retinol metabolic process; triacylglycerol biosynthetic process; triacylglycerol metabolic process; very-low-density lipoprotein particle assembly

Disease: Diarrhea 7