Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) Standard Curve.)
Mouse Deleted In Lung And Esophageal Cancer Protein 1 (DLEC1) ELISA Kit | DLEC1 elisa kit
Mouse Deleted In Lung And Esophageal Cancer Protein 1 (DLEC1) ELISA Kit
Reactivity
Mouse
Synonyms
Deleted In Lung And Esophageal Cancer Protein 1 (DLEC1); Mouse Deleted In Lung And Esophageal Cancer Protein 1 (DLEC1) ELISA Kit; DLEC1; DLC1; F56; deleted in lung cancer 1; DLEC1 elisa kit
Reactivity
Mouse
Specificity
Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLEC1. No significant cross-reactivity or interference between Mouse DLEC1 and analogues was observed.
Sequence Length
635
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) Standard Curve.)
Related Product Information for DLEC1 elisa kit
Description: This Mouse Deleted in lung and esophageal cancer protein 1 (DLEC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLEC1 in samples. An antibody specific for DLEC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLEC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLEC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLEC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLC1 contains 37 exons and spans approximately 59 kb. The predicted DLC1 protein contains 1,755 amino acids. Expression of DLC1 significantly suppressed the growth of 4 different cancer cell lines. Northern blot analysis revealed that DLC1 was expressed as 6- and 8-kb mRNAs in all tissues tested, most abundantly in prostate and testis. the 8-kb mRNA and several other alternatively spliced DLC1 transcripts contain disrupted coding regions and are likely to encode nonfunctional proteins. RT-PCR experiments indicated that 33% of primary lung, esophageal, and renal cancers and cancer cell lines tested either lacked DLC1 transcripts entirely or contained increased levels of nonfunctional DLC1 mRNA. The authors concluded that aberrant transcription of DLC1 may be involved in carcinogenesis of the lung, esophagus, and kidney.
Background: DLC1 contains 37 exons and spans approximately 59 kb. The predicted DLC1 protein contains 1,755 amino acids. Expression of DLC1 significantly suppressed the growth of 4 different cancer cell lines. Northern blot analysis revealed that DLC1 was expressed as 6- and 8-kb mRNAs in all tissues tested, most abundantly in prostate and testis. the 8-kb mRNA and several other alternatively spliced DLC1 transcripts contain disrupted coding regions and are likely to encode nonfunctional proteins. RT-PCR experiments indicated that 33% of primary lung, esophageal, and renal cancers and cancer cell lines tested either lacked DLC1 transcripts entirely or contained increased levels of nonfunctional DLC1 mRNA. The authors concluded that aberrant transcription of DLC1 may be involved in carcinogenesis of the lung, esophagus, and kidney.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Deleted in lung and esophageal cancer protein 1 homolog
UniProt Protein Name
Deleted in lung and esophageal cancer protein 1 homolog
Protein Family
UniProt Gene Name
Dlec1
UniProt Entry Name
DLEC1_MOUSE
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Product Notes
The Mouse DLEC1 dlec1 (Catalog #AAA9717867) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717867 ELISA Kit recognizes Mouse DLEC1. It is sometimes possible for the material contained within the vial of "Deleted In Lung And Esophageal Cancer Protein 1 (DLEC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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