Mouse Ddx3x/ATP-dependent RNA helicase DDX3X ELISA Kit | Ddx3x elisa kit

Mouse Ddx3x/ATP-dependent RNA helicase DDX3X ELISA Kit

Cat. Num. AAA2904778

• Gene Names: Ddx3x; Ddx3; Fin14; D1Pas1-rs2
• Reactivity: Mouse
• Synonyms: Ddx3x/ATP-dependent RNA helicase DDX3X; Mouse Ddx3x/ATP-dependent RNA helicase DDX3X ELISA Kit; D1Pas1-related sequence 2; DEAD box RNA helicase DEAD3; mDEAD3; DEAD box protein 3; X-chromosomal; Embryonic RNA helicase; D1Pas1-rs2; Ddx3; Dead3; Erh; ATP-dependent RNA helicase DDX3X; Ddx3x elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse ATP-dependent RNA helicase DDX3X

• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 6753620
• NCBI GeneID: 13205
• NCBI Accession #: NP_034158.1
• NCBI GenBank Nucleotide #: NM_010028.3
• UniProt Accession #: Q62167; O09060; O09143
• Molecular Weight: 73,101 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DDX3X
• NCBI Official Synonym Full Names: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
• NCBI Official Symbol: Ddx3x
• NCBI Official Synonym Symbols: Ddx3; Fin14; D1Pas1-rs2
• NCBI Protein Information: ATP-dependent RNA helicase DDX3X
• UniProt Protein Name: ATP-dependent RNA helicase DDX3X
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: Ddx3x
• UniProt Synonym Gene Names: D1Pas1-rs2; Ddx3; Dead3; Erh; mDEAD3
• UniProt Entry Name: DDX3X_MOUSE

Uniprot Description

DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; RNA-binding; Translation initiation; EC 3.6.4.13; Cell cycle regulation; Spliceosome

Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; membrane; mitochondrion; stress granule; cytoplasm; nucleus

Molecular Function: GTPase activity; CTPase activity; hydrolase activity; RNA binding; ATPase activity; nucleotide binding; helicase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; nucleic acid binding; translation initiation factor binding; eukaryotic initiation factor 4E binding; nucleoside-triphosphatase activity; mRNA 5'-UTR binding; ATP binding; ribosomal small subunit binding; ATP-dependent RNA helicase activity

Biological Process: stress granule assembly; positive regulation of viral genome replication; transcription, DNA-dependent; positive regulation of translation; apoptosis; positive regulation of apoptosis; immune system process; response to virus; ribosome biogenesis and assembly; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; regulation of translation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; negative regulation of translation; RNA secondary structure unwinding; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis