Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit | CRLF1 elisa kit

Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

Cat. Num. AAA2019717

• Gene Names: CRLF1; CLF; NR6; CISS; CISS1; CLF-1; zcytor5
• Reactivity: Mouse
• Synonyms: Cytokine Receptor Like Factor 1 (CRLF1); Mouse Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit; CISS; CISS1; CLF; CLF-1; NR6; Cytokine-like factor 1; ZcytoR5; CRLF1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytokine Receptor Like Factor 1 (CRLF1).; No significant cross-reactivity or interference between Cytokine Receptor Like Factor 1 (CRLF1) and analogues was observed.
• Sequence Length: 422

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokine Receptor Like Factor 1 (CRLF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokine Receptor Like Factor 1 (CRLF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRLF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokine Receptor Like Factor 1 (CRLF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokine Receptor Like Factor 1 (CRLF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CRLF1 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 4758062
• NCBI GeneID: 9244
• NCBI Accession #: NP_004741.1
• NCBI GenBank Nucleotide #: NM_004750.4
• UniProt Accession #: O75462; Q9UHH5
• Molecular Weight: 46,302 Da
• NCBI Official Full Name: cytokine receptor-like factor 1
• NCBI Official Synonym Full Names: cytokine receptor-like factor 1
• NCBI Official Symbol: CRLF1
• NCBI Official Synonym Symbols: CLF; NR6; CISS; CISS1; CLF-1; zcytor5
• NCBI Protein Information: cytokine receptor-like factor 1
• UniProt Protein Name: Cytokine receptor-like factor 1
• Protein Family: Cytokine receptor-like factor
• UniProt Gene Name: CRLF1
• UniProt Synonym Gene Names: CLF-1
• UniProt Entry Name: CRLF1_HUMAN

NCBI Description

This gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuronal cells. Mutations in this gene result in Crisponi syndrome and cold-induced sweating syndrome. [provided by RefSeq, Oct 2009]

Uniprot Description

CRLF1: Cytokine receptor subunit, possibly playing a regulatory role in the immune system and during fetal development. May be involved in nervous system development. Defects in CRLF1 are the cause of cold-induced sweating syndrome type 1 (CISS1). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Defects in CRLF1 are the cause of Crisponi syndrome (CRISPS). Crisponi syndrome is a rare autosomal recessive disorder characterized by congenital muscular contractions of facial muscles, with trismus in response to stimuli, dysmorphic features, bilateral camptodactyly, major feeding and respiratory difficulties, and access of hyperthermia leading to death in the first months of life. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: extracellular space

Molecular Function: protein binding; protein heterodimerization activity; cytokine binding; cytokine activity; receptor activity; ciliary neurotrophic factor receptor binding

Biological Process: ureteric bud development; positive regulation of cell proliferation; negative regulation of neuron apoptosis; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 1