Mouse Cytochrome P450 2E1 ELISA Kit | CYP2E1 elisa kit

Mouse Cytochrome P450 2E1 ELISA Kit

Cat. Num. AAA3807018

• Gene Names: CYP2E1; CPE1; CYP2E; P450-J; P450C2E
• Reactivity: Mouse
• Synonyms: Cytochrome P450 2E1; Mouse Cytochrome P450 2E1 ELISA Kit; CYP2E1 elisa kit

• Reactivity: Mouse
• Sequence Length: 493

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 5 U/L-80 U/L
• Sensitivity: 0.1 U/L

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2E1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCYTOCHROME P450 2E1in the sample, This CYTOCHROME P450 2E1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCYTOCHROME P450 2E1concentration. The concentration ofCYTOCHROME P450 2E1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 117250
• NCBI GeneID: 1571
• UniProt Accession #: P05181
• NCBI Official Full Name: Cytochrome P450 2E1
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily E member 1
• NCBI Official Symbol: CYP2E1
• NCBI Official Synonym Symbols: CPE1; CYP2E; P450-J; P450C2E
• NCBI Protein Information: cytochrome P450 2E1
• UniProt Protein Name: Cytochrome P450 2E1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2E1
• UniProt Synonym Gene Names: CYP2E
• UniProt Entry Name: CP2E1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Mitochondrial; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; EC 1.14.13.n7; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: intrinsic to endoplasmic reticulum membrane; Golgi membrane; endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; response to ethanol; triacylglycerol metabolic process; response to ozone; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process; response to organic nitrogen