Mouse Cyclin-D1 (CCND1) ELISA Kit | CCND1 elisa kit

Mouse Cyclin-D1 (CCND1) ELISA Kit

Cat. Num. AAA287781

• Gene Names: Ccnd1; cD1; CycD1; Cyl-1; PRAD1; bcl-1; AI327039
• Reactivity: Mouse
• Synonyms: Cyclin-D1 (CCND1); Mouse Cyclin-D1 (CCND1) ELISA Kit; BCL1; D11S287E; PRAD1; U21B31; B-cell CLL/lymphoma 1; G1/S-Specific Cyclin-D1; Parathyroid Adenomatosis 1; CCND1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CCND1. No significant cross-reactivity or interference between Mouse CCND1 and analogues was observed.
• Sequence Length: 295

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CCND1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate CCND1 in samples. An antibody specific for CCND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6680868
• NCBI GeneID: 12443
• NCBI Accession #: NP_031657.1
• NCBI GenBank Nucleotide #: NM_007631.2
• UniProt Accession #: P25322
• Molecular Weight: 33,429 Da
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: Ccnd1
• NCBI Official Synonym Symbols: cD1; CycD1; Cyl-1; PRAD1; bcl-1; AI327039
• NCBI Protein Information: G1/S-specific cyclin-D1
• UniProt Protein Name: G1/S-specific cyclin-D1
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: Ccnd1
• UniProt Synonym Gene Names: Cyl-1

Uniprot Description

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner ().