Typical Testing Data/Standard Curve (for reference only)
Mouse CRY1 ELISA Kit | CRY1 elisa kit
Mouse CRY1 (Cryptochrome 1) ELISA Kit
Reactivity
Mouse
Synonyms
CRY1; Mouse CRY1 (Cryptochrome 1) ELISA Kit; CRY1 elisa kit
Reactivity
Mouse
Specificity
This kit recognizes Mouse CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.
Sequence Length
74
Samples
Serum And Plasma Samples
Assay Type
Quantitative Competitive
Detection Range
3.12-200ng/mL
Sensitivity
1.93ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 mouse samples with low, mid range and high level CORT were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 mouse samples with low, mid range and high level CORT were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CRY1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CORT concentrations in serum and plasma samples. Please contact tech-support for other sample type detection.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Corticosterone, also known as 17-deoxycortisol, it is a steroid hormone secreted by the outer layer, or cortex, of the adrenal gland. Classed as a glucocorticoid, corticosterone helps regulate the conversion of amino acids into carbohydrates and glycogen by the liver, and helps stimulate glycogen formation in the tissues [1]. Corticosterone is similar in structure, although somewhat less potent, than the other glucocorticoids cortisol and cortisone. It is produced in response to stimulation by the pituitary substance adrenocorticotropic hormone (ACTH). In some species, but not in humans, corticosterone is the predominant glucocorticoid secreted by the adrenal. It is a precursor in the synthesis of aldosterone, another adrenal cortical steroid. The increase in corticosterone is linked to anxiety relief. This finding depends on the time at which the administration of corticosterone took place as compared to when the fear conditioning took place, corticosterone can either facilitate or interrupt conditioned fear [2].
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Corticosterone, also known as 17-deoxycortisol, it is a steroid hormone secreted by the outer layer, or cortex, of the adrenal gland. Classed as a glucocorticoid, corticosterone helps regulate the conversion of amino acids into carbohydrates and glycogen by the liver, and helps stimulate glycogen formation in the tissues [1]. Corticosterone is similar in structure, although somewhat less potent, than the other glucocorticoids cortisol and cortisone. It is produced in response to stimulation by the pituitary substance adrenocorticotropic hormone (ACTH). In some species, but not in humans, corticosterone is the predominant glucocorticoid secreted by the adrenal. It is a precursor in the synthesis of aldosterone, another adrenal cortical steroid. The increase in corticosterone is linked to anxiety relief. This finding depends on the time at which the administration of corticosterone took place as compared to when the fear conditioning took place, corticosterone can either facilitate or interrupt conditioned fear [2].
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
8,316 Da
NCBI Official Full Name
Cry1, partial
UniProt Protein Name
Cry1
Protein Family
UniProt Gene Name
cry1
UniProt Entry Name
W0GGX3_BACTU
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Product Notes
The Mouse CRY1 cry1 (Catalog #AAA2502769) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2502769 ELISA Kit recognizes Mouse CRY1. It is sometimes possible for the material contained within the vial of "CRY1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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