Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 (HSD11B2) ELISA Kit | HSD11B2 elisa kit

Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 (HSD11B2) ELISA Kit

Cat. Num. AAA7205335

• Gene Names: HSD11B2; AME; AME1; HSD2; HSD11K; SDR9C3
• Reactivity: Mouse
• Synonyms: Corticosteroid 11-beta-dehydrogenase isozyme 2 (HSD11B2); Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 (HSD11B2) ELISA Kit; HSD11B2 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This HSD11B2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HSD11B2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSD11B2 elisa kit

Principle of the assay: HSD11B2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSD11B2 antibody and an HSD11B2-HRP conjugate. The assay sample and buffer are incubated together with HSD11B2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD11B2 concentration since HSD11B2 from samples and HSD11B2-HRP conjugate compete for the anti-HSD11B2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD11B2 from the sample, fewer sites are left to bind HSD11B2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD11B2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119392083
• NCBI GeneID: 3291
• NCBI Accession #: NP_000187.3
• NCBI GenBank Nucleotide #: NM_000196.3
• Molecular Weight: 44,127 Da
• NCBI Official Full Name: corticosteroid 11-beta-dehydrogenase isozyme 2
• NCBI Official Synonym Full Names: hydroxysteroid (11-beta) dehydrogenase 2
• NCBI Official Symbol: HSD11B2
• NCBI Official Synonym Symbols: AME; AME1; HSD2; HSD11K; SDR9C3
• NCBI Protein Information: corticosteroid 11-beta-dehydrogenase isozyme 2; 11-DH2; 11-beta-HSD2; -HSD11 type II; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 9C member 3
• UniProt Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 2
• Protein Family: Corticosteroid 11-beta-dehydrogenase isozyme
• UniProt Gene Name: HSD11B2
• UniProt Synonym Gene Names: HSD11K; 11-DH2; 11-beta-HSD2; -HSD11 type II; 11-beta-HSD
• UniProt Entry Name: DHI2_HUMAN

NCBI Description

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]

Uniprot Description

HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - C21-steroid hormone; EC 1.1.1.-; Oxidoreductase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: endoplasmic reticulum

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NAD binding; steroid binding

Biological Process: response to drug; response to food; response to glucocorticoid stimulus; response to hypoxia; aldosterone mediated regulation of blood volume; female pregnancy; glucocorticoid biosynthetic process; response to insulin stimulus

Disease: Apparent Mineralocorticoid Excess