Mouse CollagenType II Alpha 1 (COL2a1) ELISA Kit | COL2a1 elisa kit

Mouse CollagenType II Alpha 1 (COL2a1) ELISA Kit

Cat. Num. AAA457003

• Gene Names: Col2a1; Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; Rgsc856
• Reactivity: Mouse
• Synonyms: CollagenType II Alpha 1 (COL2a1); Mouse CollagenType II Alpha 1 (COL2a1) ELISA Kit; COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis; Spondyloepiphyseal Dysplasia; Congenital; COL2a1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL2a1. No significant cross-reactivity or interference between COL2a1 and analogues was observed.
• Sequence Length: 1419

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.055ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL2a1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL2a1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL2a1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of COL2a1 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL2a1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL2a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL2a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL2a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 169658375
• NCBI GeneID: 12824
• NCBI Accession #: NP_001106987.2
• NCBI GenBank Nucleotide #: NM_001113515.2
• UniProt Accession #: P28481; Q61428; Q62031; Q62032; Q62033; Q641K3; Q6LDB1; Q6LDI8; Q6LDI9; Q80VY3; Q80X38; Q8CEF7
• Molecular Weight: 134,722 Da
• NCBI Official Full Name: collagen alpha-1(II) chain isoform 2
• NCBI Official Synonym Full Names: collagen, type II, alpha 1
• NCBI Official Symbol: Col2a1
• NCBI Official Synonym Symbols: Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; Rgsc856
• NCBI Protein Information: collagen alpha-1(II) chain
• UniProt Protein Name: Collagen alpha-1(II) chain
• UniProt Gene Name: Col2a1

NCBI Description

This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]

Uniprot Description

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.