Mouse Collagen Type IV Alpha 1 (COL4a1) ELISA Kit | COL4a1 elisa kit

Mouse Collagen Type IV Alpha 1 (COL4a1) ELISA Kit

Cat. Num. AAA454208

• Gene Names: Col4a1; Bru; Raw; Svc; Col4a-1
• Reactivity: Mouse
• Synonyms: Collagen Type IV Alpha 1 (COL4a1); Mouse Collagen Type IV Alpha 1 (COL4a1) ELISA Kit; COL4-A1; Arresten; Collagen Alpha-1(IV)chain; COL4a1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL4a1. No significant cross-reactivity or interference between COL4a1 and analogues was observed.
• Sequence Length: 1669

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4a1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4a1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4a1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4a1 in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL4a1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL4a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL4a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161484654
• NCBI GeneID: 12826
• NCBI Accession #: NP_034061.2
• NCBI GenBank Nucleotide #: NM_009931.2
• UniProt Accession #: P02463; Q3UHJ4; Q3UJE7; Q3UQV2; Q53X35; Q6GQS7; Q6PHB5; Q99LQ8
• Molecular Weight: 160,679 Da
• NCBI Official Full Name: collagen alpha-1(IV) chain
• NCBI Official Synonym Full Names: collagen, type IV, alpha 1
• NCBI Official Symbol: Col4a1
• NCBI Official Synonym Symbols: Bru; Raw; Svc; Col4a-1
• NCBI Protein Information: collagen alpha-1(IV) chain
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: Col4a1

NCBI Description

This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin ().