Mouse Chordin Like Protein 2 (CHRDL2) ELISA Kit | CHRDL2 elisa kit

Mouse Chordin Like Protein 2 (CHRDL2) ELISA Kit

Cat. Num. AAA2705872

• Gene Names: CHRDL2; BNF1; CHL2
• Reactivity: Mouse
• Synonyms: Chordin Like Protein 2 (CHRDL2); Mouse Chordin Like Protein 2 (CHRDL2) ELISA Kit; BNF1; CHL2; Breast Tumor Novel Factor 1; Chordin-related protein 2; CHRDL2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chordin Like Protein 2 (CHRDL2).; No significant cross-reactivity or interference between Chordin Like Protein 2 (CHRDL2) and analogues was observed.
• Sequence Length: 93

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chordin Like Protein 2 (CHRDL2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chordin Like Protein 2 (CHRDL2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRDL2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin Like Protein 2 (CHRDL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin Like Protein 2 (CHRDL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin Like Protein 2 (CHRDL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chordin Like Protein 2 (CHRDL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 510937050
• NCBI GeneID: 25884
• NCBI Accession #: NP_001265402.1
• NCBI GenBank Nucleotide #: NM_001278473.2
• UniProt Accession #: Q6WN34; Q6WN30; Q6WN31; Q6WN32; Q7Z5J3; A5PKU9
• Molecular Weight: 9,846 Da
• NCBI Official Full Name: chordin-like protein 2 isoform 2
• NCBI Official Synonym Full Names: chordin like 2
• NCBI Official Symbol: CHRDL2
• NCBI Official Synonym Symbols: BNF1; CHL2
• NCBI Protein Information: chordin-like protein 2
• UniProt Protein Name: Chordin-like protein 2
• Protein Family: Chordin-like protein
• UniProt Gene Name: CHRDL2
• UniProt Synonym Gene Names: BNF1; CHL2; BNF-1

NCBI Description

This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration. [provided by RefSeq, Jan 2015]

Uniprot Description

May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation (). Implicated in tumor angiogenesis. May play a role during myoblast and osteoblast differentiation, and maturation.