Typical Testing Data/Standard Curve (for reference only)
Mouse Cholesterol 24-hydroxylase (CYP46A1) ELISA Kit | CYP46A1 elisa kit
Mouse Cholesterol 24-hydroxylase (CYP46A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CYP46A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYP46A1 antibody and an CYP46A1-HRP conjugate. The assay sample and buffer are incubated together with CYP46A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP46A1 concentration since CYP46A1 from samples and CYP46A1-HRP conjugate compete for the anti-CYP46A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP46A1 from the sample, fewer sites are left to bind CYP46A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP46A1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP46A1: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.98; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q32.1
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane
Molecular Function: iron ion binding; cholesterol 24-hydroxylase activity; heme binding; steroid hydroxylase activity
Biological Process: bile acid biosynthetic process; nervous system development; bile acid metabolic process; xenobiotic metabolic process; cholesterol catabolic process; sterol metabolic process
Research Articles on CYP46A1
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Product Notes
The Mouse CYP46A1 cyp46a1 (Catalog #AAA7235102) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235102 ELISA Kit recognizes Mouse CYP46A1. It is sometimes possible for the material contained within the vial of "Cholesterol 24-hydroxylase (CYP46A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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