Mouse Chemokine (C-X-C Motif) Ligand 27 (CCL27) ELISA Kit | CCL27 elisa kit

Mouse Chemokine (C-X-C Motif) Ligand 27 (CCL27) Mini Samples ELISA Kit

Cat. Num. AAA2708361

• Gene Names: CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Chemokine (C-X-C Motif) Ligand 27 (CCL27); Mouse Chemokine (C-X-C Motif) Ligand 27 (CCL27) Mini Samples ELISA Kit; Chemokine (C-X-C Motif) Ligand 27; CTACK; ILC; SCYA27; ALP; Skinkine; ESkine; PESKY; CTAK; Cutaneous T-Cell Attracting Chemokine; Small Inducible Cytokine Subfamily A Member 27; IL-11 R-alpha-locus chemokine; CCL27 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27).; No significant cross-reactivity or interference between Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27) and analogues was observed.
• Sequence Length: 113

• Applicable Applications for CCL27 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Chemokine (C-X-C Motif) Ligand 27 (CCL27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 4204910
• NCBI GeneID: 6359
• NCBI Official Full Name: chemokine
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 15
• NCBI Official Symbol: CCL15
• NCBI Official Synonym Symbols: LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• NCBI Protein Information: C-C motif chemokine 15
• Protein Family: C-C motif chemokine

NCBI Description

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]