Human Chemokine C-C-Motif Receptor 1 (CCR1) RTU ELISA Kit | CCR1 rtu elisa kit

Human Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit

Cat. Num. AAA458202

• Gene Names: CCR1; CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Receptor 1 (CCR1); Human Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit; CD191; CMKBR1; HM145; MIP1aR; SCYAR1; RANTES-R; Macrophage inflammatory protein 1-alpha receptor; LD78 receptor; CCR1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 355

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.071ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502631
• NCBI GeneID: 1230
• NCBI Accession #: NP_001286.1
• NCBI GenBank Nucleotide #: NM_001295.2
• UniProt Accession #: P32246; Q86VA9
• Molecular Weight: 41,173 Da
• NCBI Official Full Name: C-C chemokine receptor type 1
• NCBI Official Synonym Full Names: C-C motif chemokine receptor 1
• NCBI Official Symbol: CCR1
• NCBI Official Synonym Symbols: CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• NCBI Protein Information: C-C chemokine receptor type 1
• UniProt Protein Name: C-C chemokine receptor type 1
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR1
• UniProt Synonym Gene Names: CMKBR1; CMKR1; SCYAR1; C-C CKR-1; CC-CKR-1; CCR-1; CCR1; MIP-1alpha-R

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.