Mouse CCKAR ELISA Kit | CCKAR elisa kit

Mouse CCKAR (Cholecystokinin A Receptor) ELISA Kit

Cat. Num. AAA2506358

• Gene Names: CCKAR; CCK-A; CCK1R; CCKRA; CCK1-R
• Reactivity: Mouse
• Synonyms: CCKAR; Mouse CCKAR (Cholecystokinin A Receptor) ELISA Kit; CCKAR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CCKAR. No significant cross-reactivity or interference between Mouse CCKAR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CCKAR and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 428

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCKAR elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCKAR in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCKAR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CCKAR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCKAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CCKAR. You can calculate the concentration of Mouse CCKAR in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502607
• NCBI GeneID: 886
• NCBI Accession #: NP_000721.1
• NCBI GenBank Nucleotide #: NM_000730.2
• UniProt Accession #: P32238; B2R9Z5
• Molecular Weight: 47,841 Da
• NCBI Official Full Name: cholecystokinin receptor type A
• NCBI Official Synonym Full Names: cholecystokinin A receptor
• NCBI Official Symbol: CCKAR
• NCBI Official Synonym Symbols: CCK-A; CCK1R; CCKRA; CCK1-R
• NCBI Protein Information: cholecystokinin receptor type A; CCK-A receptor; CCK-AR; cholecystokinin type-A receptor; cholecystokinin-1 receptor
• UniProt Protein Name: Cholecystokinin receptor type A
• Protein Family: Cholecystokinin receptor
• UniProt Gene Name: CCKAR
• UniProt Synonym Gene Names: CCKRA; CCK-A receptor; CCK-AR; CCK1-R
• UniProt Entry Name: CCKAR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008]

Uniprot Description

CCKAR: Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: cholecystokinin receptor activity; peptide binding

Biological Process: elevation of cytosolic calcium ion concentration; axonogenesis; digestion; forebrain development; regulation of hormone secretion; neuron migration; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); response to nutrient; cellular response to hormone stimulus