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Typical Testing Data/Standard Curve (for reference only)

Mouse Carboxypeptidase D (CPD) ELISA Kit | CPD elisa kit

Mouse Carboxypeptidase D (CPD) ELISA Kit

Gene Names
CPD; GP180
Reactivity
Mouse
Synonyms
Carboxypeptidase D (CPD); Mouse Carboxypeptidase D (CPD) ELISA Kit; CPD elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CPD. No significant cross-reactivity or interference between CPD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPD and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CPD elisa kit
Intended Uses: This CPD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CPD. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CPD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CPD antibody and an CPD-HRP conjugate. The assay sample and buffer are incubated together with CPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPD concentration since CPD from samples and CPD-HRP conjugate compete for the anti-CPD antibody binding site. Since the number of sites is limited, as more sites are occupied by CPD from the sample, fewer sites are left to bind CPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPD concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,487 Da
NCBI Official Full Name
carboxypeptidase D isoform 1
NCBI Official Synonym Full Names
carboxypeptidase D
NCBI Official Symbol
CPD
NCBI Official Synonym Symbols
GP180
NCBI Protein Information
carboxypeptidase D; metallocarboxypeptidase D
UniProt Protein Name
Carboxypeptidase D
Protein Family
UniProt Gene Name
CPD
UniProt Entry Name
CBPD_HUMAN

NCBI Description

The metallocarboxypeptidase family of enzymes is divided into 2 subfamilies based on sequence similarities. The pancreatic carboxypeptidase-like and the regulatory B-type carboxypeptidase subfamilies. Carboxypeptidase D has been identified as a regulatory B-type carboxypeptidase. CPD is a homolog of duck gp180, a hepatitis B virus-binding protein. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPD: The metallocarboxypeptidase family of enzymes is divided into 2 subfamilies based on sequence similarities. The pancreatic carboxypeptidase-like and the regulatory B-type carboxypeptidase subfamilies. Carboxypeptidase D has been identified as a regulatory B-type carboxypeptidase. CPD is a homolog of duck gp180, a hepatitis B virus-binding protein. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Jul 2008]

Protein type: Protease; Membrane protein, integral; EC 3.4.17.22

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: membrane; integral to membrane

Molecular Function: serine carboxypeptidase activity; zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis

Research Articles on CPD

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Product Notes

The Mouse CPD cpd (Catalog #AAA7209873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209873 ELISA Kit recognizes Mouse CPD. It is sometimes possible for the material contained within the vial of "Carboxypeptidase D (CPD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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