Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse C-X-C motif chemokine 13 (CXCL13) Standard Curve.)
Mouse C-X-C Motif Chemokine 13 (CXCL13) ELISA Kit | CXCL13 elisa kit
Mouse C-X-C Motif Chemokine 13 (CXCL13) ELISA Kit
Gene Names
Cxcl13; BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik
Reactivity
Mouse
Synonyms
C-X-C Motif Chemokine 13 (CXCL13); Mouse C-X-C Motif Chemokine 13 (CXCL13) ELISA Kit; CXCL13; ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt s lymphoma receptor-1); B-lymphocyte chemoattractant; c; CXCL13 elisa kit
Reactivity
Mouse
Specificity
Mouse C-X-C motif chemokine 13 (CXCL13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CXCL13. No significant cross-reactivity or interference between Mouse CXCL13 and analogues was observed.
Sequence Length
109
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse C-X-C motif chemokine 13 (CXCL13) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse C-X-C motif chemokine 13 (CXCL13) Standard Curve.)
Related Product Information for CXCL13 elisa kit
Description: This Mouse C-X-C motif chemokine 13 (CXCL13) ELISA Kit employs a two-site sandwich ELISA to quantitate CXCL13 in samples. An antibody specific for CXCL13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles.
Background: B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-X-C motif chemokine 13
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 13
NCBI Official Symbol
Cxcl13
NCBI Official Synonym Symbols
BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik
NCBI Protein Information
C-X-C motif chemokine 13
UniProt Protein Name
C-X-C motif chemokine 13
Protein Family
UniProt Gene Name
Cxcl13
UniProt Synonym Gene Names
Blc; Scyb13
UniProt Entry Name
CXL13_MOUSE
Uniprot Description
CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: extracellular region; extracellular space
Molecular Function: CCR10 chemokine receptor binding; chemokine activity; CXCR3 chemokine receptor binding; CXCR5 chemokine receptor binding; fibroblast growth factor binding; heparin binding; protein heterodimerization activity; receptor agonist activity
Biological Process: cell surface receptor linked signal transduction; cell-cell signaling; defense response to bacterium; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymph node development; lymphocyte chemotaxis across high endothelial venule; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; positive regulation of leukocyte chemotaxis; regulation of cell proliferation; response to lipopolysaccharide
Research Articles on CXCL13
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Product Notes
The Mouse CXCL13 cxcl13 (Catalog #AAA9717976) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717976 ELISA Kit recognizes Mouse CXCL13. It is sometimes possible for the material contained within the vial of "C-X-C Motif Chemokine 13 (CXCL13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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