Typical Testing Data/Standard Curve (for reference only)
Mouse C-type lectin domain family 5 member A (CLEC5A) ELISA Kit | CLEC5A elisa kit
Mouse C-type lectin domain family 5 member A (CLEC5A) ELISA Kit
Gene Names
CLEC5A; CLECSF5
Reactivity
Mouse
Synonyms
C-type lectin domain family 5 member A (CLEC5A); Mouse C-type lectin domain family 5 member A (CLEC5A) ELISA Kit; CLEC5A elisa kit
Reactivity
Mouse
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CLEC5A elisa kit
Intended Uses: This CLEC5A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC5A. This ELISA kit for research use only!
Principle of the Assay: CLEC5A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC5A antibody and an CLEC5A-HRP conjugate. The assay sample and buffer are incubated together with CLEC5A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC5A concentration since CLEC5A from samples and CLEC5A-HRP conjugate compete for the anti-CLEC5A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC5A from the sample, fewer sites are left to bind CLEC5A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC5A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CLEC5A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC5A antibody and an CLEC5A-HRP conjugate. The assay sample and buffer are incubated together with CLEC5A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC5A concentration since CLEC5A from samples and CLEC5A-HRP conjugate compete for the anti-CLEC5A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC5A from the sample, fewer sites are left to bind CLEC5A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC5A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC5A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,653 Da
NCBI Official Full Name
C-type lectin domain family 5 member A
NCBI Official Symbol
CLEC5A
NCBI Official Synonym Symbols
CLECSF5
NCBI Protein Information
C-type lectin domain family 5 member A; MDL-1; C-type lectin superfamily member 5; myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin long form
UniProt Protein Name
C-type lectin domain family 5 member A
Protein Family
UniProt Gene Name
CLEC5A
UniProt Synonym Gene Names
CLECSF5; MDL1; MDL-1
UniProt Entry Name
CLC5A_PIG
Uniprot Description
Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation ().
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Product Notes
The Mouse CLEC5A clec5a (Catalog #AAA7249150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249150 ELISA Kit recognizes Mouse CLEC5A. It is sometimes possible for the material contained within the vial of "C-type lectin domain family 5 member A (CLEC5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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