Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bone morphogenetic protein 6 (BMP-6) Standard Curve.)
Mouse Bone Morphogenetic Protein 6 (BMP-6) ELISA Kit | BMP-6 elisa kit
Mouse Bone Morphogenetic Protein 6 (BMP-6) ELISA Kit
Gene Names
Bmp6; Vgr1; D13Wsu115e
Reactivity
Mouse
Synonyms
Bone Morphogenetic Protein 6 (BMP-6); Mouse Bone Morphogenetic Protein 6 (BMP-6) ELISA Kit; BMP6; VGR; VGR1; Vg1-related sequence; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; BMP-6 elisa kit
Reactivity
Mouse
Specificity
Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse BMP6. No significant cross-reactivity or interference between Mouse BMP6 and analogues was observed.
Sequence Length
510
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bone morphogenetic protein 6 (BMP-6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bone morphogenetic protein 6 (BMP-6) Standard Curve.)
Related Product Information for BMP-6 elisa kit
Description: This Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate BMP6 in samples. An antibody specific for BMP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Bone morphogenetic proteins, such as BMP6, belong to the transforming growth factor-beta superfamily of regulatory molecules (Rickard et al., 1998).Rickard et al. (1998) presented evidence that the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP6 by osteoblasts. They investigated the effect of estrogen on BMP production in 2 estrogen-responsive, human immortalized cell lines that display the mature osteoblast phenotype.Tamada et al. (1998) found that the promoter region of the BMP6 gene lacks a canonical TATA box, but has a GC-rich region with 2 SP1-binding sites, an inverted CCAAT element, and a putative tramtrack responsive element. Reporter gene assays suggested that the BMP6 promoter has osteogenic cell specificity.
Background: Bone morphogenetic proteins, such as BMP6, belong to the transforming growth factor-beta superfamily of regulatory molecules (Rickard et al., 1998).Rickard et al. (1998) presented evidence that the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP6 by osteoblasts. They investigated the effect of estrogen on BMP production in 2 estrogen-responsive, human immortalized cell lines that display the mature osteoblast phenotype.Tamada et al. (1998) found that the promoter region of the BMP6 gene lacks a canonical TATA box, but has a GC-rich region with 2 SP1-binding sites, an inverted CCAAT element, and a putative tramtrack responsive element. Reporter gene assays suggested that the BMP6 promoter has osteogenic cell specificity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone morphogenetic protein 6 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 6
NCBI Official Symbol
Bmp6
NCBI Official Synonym Symbols
Vgr1; D13Wsu115e
NCBI Protein Information
bone morphogenetic protein 6
UniProt Protein Name
Bone morphogenetic protein 6
Protein Family
UniProt Gene Name
Bmp6
UniProt Synonym Gene Names
Bmp-6; Vgr1; BMP-6; VGR-1
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates a wide range of biological processes including iron homeostasis, fat and bone development, and ovulation. Mice lacking this gene exhibit delayed ossification of the sternum, iron overload, and reduced fertility in females. [provided by RefSeq, Jul 2016]
Uniprot Description
Induces cartilage and bone formation.
Research Articles on BMP-6
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Product Notes
The Mouse BMP-6 bmp6 (Catalog #AAA9718158) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718158 ELISA Kit recognizes Mouse BMP-6. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 6 (BMP-6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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