Typical Testing Data/Standard Curve (for reference only)
Mouse Bisphosphoglycee mutase (BPGM) ELISA Kit | BPGM elisa kit
Mouse Bisphosphoglycee mutase (BPGM) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
Uniprot Description
BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
Protein type: Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.4.2.4; Phosphatase (non-protein); EC 5.4.2.11; EC 3.1.3.13
Chromosomal Location of Human Ortholog: 7q33
Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity
Biological Process: erythrocyte development; glycolysis; dephosphorylation; carbohydrate metabolic process; respiratory gaseous exchange
Disease: Bisphosphoglycerate Mutase Deficiency
Research Articles on BPGM
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Product Notes
The Mouse BPGM bpgm (Catalog #AAA7209672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209672 ELISA Kit recognizes Mouse BPGM. It is sometimes possible for the material contained within the vial of "Bisphosphoglycee mutase (BPGM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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