Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bile acyl-CoA synthetase (SLC27A5) Standard Curve.)
Mouse Bile Acyl-CoA Synthetase (SLC27A5) ELISA Kit | SLC27A5 elisa kit
Mouse Bile Acyl-CoA Synthetase (SLC27A5) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bile acyl-CoA synthetase (SLC27A5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Bile acyl-CoA synthetase (SLC27A5) Standard Curve.)
Background: Very long chain fatty acid synthetase (VLACS, or SLC27A2), an enzyme whose function is presumed missing in ALD, is highly expressed in liver and kidney, but is expressed at much lower levels in brain. However, brain contains high amounts of VLCFA and is the organ most affected in ALD. In an attempt to isolate a VLACS isoform with a different tissue distribution, Berger et al. (1998) used degenerate PCR to clone a mouse cDNA and a partial human cDNA encoding a VLACS-related protein, termed VLACSR. VLACSR is 43% identical to mouse VLACS and 37% identical to mouse FATP. Northern blot analysis of mouse tissues revealed that a 2.6-kb VLACSR mRNA was highly abundant in liver. Smaller VLACSR transcripts were present at low levels in brain, lung, testis, spleen, and skeletal muscle.
NCBI and Uniprot Product Information
Uniprot Description
SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; EC 6.2.1.7; Ligase; Membrane protein, multi-pass
Cellular Component: basal plasma membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; protein complex
Molecular Function: ATP binding; catalytic activity; cholate-CoA ligase activity; fatty acid transporter activity; ligase activity; long-chain-fatty-acid-CoA ligase activity; nucleotide binding; protein complex binding; very-long-chain-fatty-acid-CoA ligase activity
Biological Process: bile acid biosynthetic process; bile acid metabolic process; fatty acid metabolic process; fatty acid transport; ketone body biosynthetic process; lipid metabolic process; long-chain fatty acid metabolic process; metabolic process; plasma membrane long-chain fatty acid transport; triacylglycerol mobilization; very-long-chain fatty acid metabolic process
Research Articles on SLC27A5
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Product Notes
The Mouse SLC27A5 slc27a5 (Catalog #AAA9717038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717038 ELISA Kit recognizes Mouse SLC27A5. It is sometimes possible for the material contained within the vial of "Bile Acyl-CoA Synthetase (SLC27A5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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