Typical Testing Data/Standard Curve (for reference only)
Human Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (GCNT3) ELISA Kit | GCNT3 elisa kit
Human Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (GCNT3) ELISA Kit
Gene Names
GCNT3; GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
Reactivity
Human
Synonyms
Beta-1; 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase 3 (GCNT3); Human Beta-1; 6-N-acetylglucosaminyltransferase 3 (GCNT3) ELISA Kit; GCNT3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GCNT3. No significant cross-reactivity or interference between GCNT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCNT3 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
438
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GCNT3 elisa kit
Intended Uses: This GCNT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GCNT3. This ELISA kit for research use only!
Principle of the Assay: GCNT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GCNT3 antibody and an GCNT3-HRP conjugate. The assay sample and buffer are incubated together with GCNT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCNT3 concentration since GCNT3 from samples and GCNT3-HRP conjugate compete for the anti-GCNT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCNT3 from the sample, fewer sites are left to bind GCNT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCNT3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GCNT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GCNT3 antibody and an GCNT3-HRP conjugate. The assay sample and buffer are incubated together with GCNT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCNT3 concentration since GCNT3 from samples and GCNT3-HRP conjugate compete for the anti-GCNT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCNT3 from the sample, fewer sites are left to bind GCNT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCNT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,864 Da
NCBI Official Full Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
NCBI Official Synonym Full Names
glucosaminyl (N-acetyl) transferase 3, mucin type
NCBI Official Symbol
GCNT3
NCBI Official Synonym Symbols
GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
NCBI Protein Information
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Protein Name
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Gene Name
GCNT3
UniProt Synonym Gene Names
C2GnT-M; hC2GnT-M; C2/4GnT
NCBI Description
This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]
Uniprot Description
Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Research Articles on GCNT3
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Product Notes
The Human GCNT3 gcnt3 (Catalog #AAA7253949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7253949 ELISA Kit recognizes Human GCNT3. It is sometimes possible for the material contained within the vial of "Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (GCNT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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