Typical Testing Data/Standard Curve (for reference only)
Mouse Bcl-2-related protein A1 (BCL2A1) ELISA Kit | BCL2A1 elisa kit
Mouse Bcl-2-related protein A1 (BCL2A1) ELISA Kit
Gene Names
Bcl2a1a; A1; Bfl-1; Hbpa1; Bcl2a1; BB218357
Reactivity
Mouse
Synonyms
Bcl-2-related protein A1 (BCL2A1); Mouse Bcl-2-related protein A1 (BCL2A1) ELISA Kit; BCL2A1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of BCL2A1. No significant cross-reactivity or interference between BCL2A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL2A1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BCL2A1 elisa kit
Intended Uses: This BCL2A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BCL2A1. This ELISA kit for research use only!
Principle of the Assay: BCL2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCL2A1 antibody and an BCL2A1-HRP conjugate. The assay sample and buffer are incubated together with BCL2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2A1 concentration since BCL2A1 from samples and BCL2A1-HRP conjugate compete for the anti-BCL2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2A1 from the sample, fewer sites are left to bind BCL2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2A1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BCL2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCL2A1 antibody and an BCL2A1-HRP conjugate. The assay sample and buffer are incubated together with BCL2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2A1 concentration since BCL2A1 from samples and BCL2A1-HRP conjugate compete for the anti-BCL2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2A1 from the sample, fewer sites are left to bind BCL2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BCL2A1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,914 Da
NCBI Official Full Name
bcl-2-related protein A1
NCBI Official Synonym Full Names
B cell leukemia/lymphoma 2 related protein A1a
NCBI Official Symbol
Bcl2a1a
NCBI Official Synonym Symbols
A1; Bfl-1; Hbpa1; Bcl2a1; BB218357
NCBI Protein Information
bcl-2-related protein A1; A1-A; protein BFL-1; hemopoietic-specific early response protein; B-cell leukemia/lymphoma 2 related protein A1a
UniProt Protein Name
Bcl-2-related protein A1
Protein Family
UniProt Gene Name
Bcl2a1
UniProt Synonym Gene Names
A1; Bcl2a1a; Bfl1
UniProt Entry Name
B2LA1_MOUSE
Uniprot Description
BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection
Cellular Component: mitochondrial outer membrane; cytoplasm
Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity
Biological Process: regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; B cell homeostasis; apoptosis; negative regulation of B cell apoptosis; negative regulation of apoptosis
Research Articles on BCL2A1
Similar Products
Product Notes
The Mouse BCL2A1 bcl2a1 (Catalog #AAA7212897) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212897 ELISA Kit recognizes Mouse BCL2A1. It is sometimes possible for the material contained within the vial of "Bcl-2-related protein A1 (BCL2A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
