Typical Testing Data/Standard Curve (for reference only)
Human Bcl 2 related ovarian killer protein (BOK) ELISA Kit | BOK elisa kit
Human Bcl 2 related ovarian killer protein (BOK) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BOK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BOK antibody and anBOK-HRP conjugate. The assay sample and buffer are incubated together withBOK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theBOK concentration sinceBOK from samples andBOK-HRP conjugate compete for the anti-BOK antibody binding site. Since the number of sites is limited, as more sites are occupied byBOK from the sample, fewer sites are left to bindBOK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. TheBOK concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq, Sep 2011]
Uniprot Description
BOK: May play a role in apoptosis. Belongs to the Bcl-2 family.
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; cytoplasm; integral to membrane; nucleus
Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity
Biological Process: caspase activation; cell proliferation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; male gonad development; oligodendrocyte differentiation; brain development
Research Articles on BOK
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Product Notes
The Human BOK bok (Catalog #AAA7247904) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7247904 ELISA Kit recognizes Human BOK. It is sometimes possible for the material contained within the vial of "Bcl 2 related ovarian killer protein (BOK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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