Mouse Band 4.1-like protein 5 (EPB41L5) ELISA Kit | EPB41L5 elisa kit

Mouse Band 4.1-like protein 5 (EPB41L5) ELISA Kit

Cat. Num. AAA7201470

• Gene Names: Epb4.1l5; Epb41l5
• Reactivity: Mouse
• Synonyms: Band 4.1-like protein 5 (EPB41L5); Mouse Band 4.1-like protein 5 (EPB41L5) ELISA Kit; EPB41L5 elisa kit

• Reactivity: Mouse

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPB41L5 elisa kit

Intended Uses: This EPB41L3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EPB41L3. This ELISA kit for research use only!

Principle of the Assay: EPB41L3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-EPB41L3 antibody and an EPB41L3-HRP conjugate. The assay sample and buffer are incubated together with EPB41L3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPB41L3 concentration since EPB41L3 from samples and EPB41L3-HRP conjugate compete for the anti-EPB41L3 antibody binding site. Since the number of sites is limited, as more sites are occupied by EPB41L3 from the sample, fewer sites are left to bind EPB41L3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPB41L3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for EPB41L5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 58865622
• NCBI GeneID: 304733
• NCBI Accession #: NP_001012023.1
• NCBI GenBank Nucleotide #: NM_001012023.1
• Molecular Weight: 57,551 Da
• NCBI Official Full Name: band 4.1-like protein 5
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.1-like 5
• NCBI Official Symbol: Epb4.1l5
• NCBI Official Synonym Symbols: Epb41l5
• NCBI Protein Information: band 4.1-like protein 5; erythrocyte protein band 4.1-like 5; erythrocyte membrane protein band 4.1 like 5
• UniProt Protein Name: Band 4.1-like protein 5
• Protein Family: Band 4.1-like protein
• UniProt Gene Name: Epb41l5
• UniProt Synonym Gene Names: Epb4.1l5
• UniProt Entry Name: E41L5_RAT

Uniprot Description

EPB41L5: May contribute to the correct positioning of tight junctions during the establishment of polarity in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: extrinsic to membrane; cytoskeleton; focal adhesion; leading edge; cytoplasm; plasma membrane; nucleus

Molecular Function: protein domain specific binding; cytoskeletal protein binding

Biological Process: paraxial mesoderm development; unidimensional cell growth; cellular morphogenesis during differentiation; somitogenesis; positive regulation of protein binding; substrate-bound cell migration, cell attachment to substrate; neural plate morphogenesis; in utero embryonic development; actomyosin structure organization and biogenesis; mesoderm migration; somite rostral/caudal axis specification; positive regulation of focal adhesion formation; axial mesoderm morphogenesis; axial mesoderm development; embryonic foregut morphogenesis; ectoderm development; epithelial to mesenchymal transition; mesoderm development; negative regulation of cell-cell adhesion; positive regulation of cell-matrix adhesion; actin cytoskeleton organization and biogenesis; negative regulation of protein binding; endoderm development