Typical Testing Data/Standard Curve (for reference only)
Mouse BAG family molecular chaperone regulator 5 (BAG5) ELISA Kit | BAG5 elisa kit
Mouse BAG family molecular chaperone regulator 5 (BAG5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BAFF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BAFF antibody and an BAFF-HRP conjugate. The assay sample and buffer are incubated together with BAFF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAFF concentration since BAFF from samples and BAFF-HRP conjugate compete for the anti-BAFF antibody binding site. Since the number of sites is limited, as more sites are occupied by BAFF from the sample, fewer sites are left to bind BAFF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAFF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
BAG5: Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2. May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: mitochondrion; membrane; perinuclear region of cytoplasm; inclusion body; nucleus
Molecular Function: protein binding; ubiquitin protein ligase binding; chaperone binding; protein kinase binding
Biological Process: protein folding; negative regulation of ubiquitin-protein ligase activity; negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process
Research Articles on BAG5
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Product Notes
The Mouse BAG5 bag5 (Catalog #AAA7206030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206030 ELISA Kit recognizes Mouse BAG5. It is sometimes possible for the material contained within the vial of "BAG family molecular chaperone regulator 5 (BAG5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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