Typical Testing Data/Standard Curve (for reference only)
Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1) ELISA Kit | LAB7-1 elisa kit
Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1)(Sandwich ELISA) ELISA Kit
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
ELISA
Synonyms
B-Lymphocyte Activation Antigen B7-1 (LAB7-1); Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1)(Sandwich ELISA) ELISA Kit; ELISA Kit for B-Lymphocyte Activation Antigen B7-1 (LAB7-1); LAB7-1/B-Lymphocyte Activation Antigen B7-1; CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1; LAB7-1 elisa kit
Reactivity
Mouse
Specificity
Reactive with Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-1 (LAB7-1). No significant cross-reactivity or interference between B-Lymphocyte Activation Antigen B7-1 (LAB7-1) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-1 (LAB7-1). No significant cross-reactivity or interference between B-Lymphocyte Activation Antigen B7-1 (LAB7-1) and analogues was observed.
Applicable Applications for LAB7-1 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-1 (LAB7-1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-1 (LAB7-1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-1 (LAB7-1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 1 (PLCg1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase C Gamma 1 (PLCg1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 1 (PLCg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase C Gamma 1 (PLCg1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for LAB7-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,051 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 antigen
NCBI Official Symbol
Cd80
NCBI Official Synonym Symbols
B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
NCBI Protein Information
T-lymphocyte activation antigen CD80
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Gene Name
Cd80
UniProt Synonym Gene Names
B7; B7
Uniprot Description
CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16 B4|16 26.86 cM
Cellular Component: cell surface; external side of plasma membrane
Molecular Function: protein binding
Biological Process: positive regulation of alpha-beta T cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell proliferation; T cell costimulation
Research Articles on LAB7-1
Similar Products
Product Notes
The Mouse LAB7-1 cd80 (Catalog #AAA2125241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125241 ELISA Kit recognizes Mouse LAB7-1. AAA Biotech's B-Lymphocyte Activation Antigen B7-1 (LAB7-1) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the LAB7-1 cd80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-Lymphocyte Activation Antigen B7-1 (LAB7-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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