Mouse ATP Binding Cassette Transporter A9 (ABCA9) ELISA Kit | ABCA9 elisa kit

Mouse ATP Binding Cassette Transporter A9 (ABCA9) ELISA Kit

Cat. Num. AAA2025014

• Gene Names: ABCA9; EST640918
• Reactivity: Mouse
• Synonyms: ATP Binding Cassette Transporter A9 (ABCA9); Mouse ATP Binding Cassette Transporter A9 (ABCA9) ELISA Kit; ABC-A9; ABCA9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A9 (ABCA9).; No significant cross-reactivity or interference between ATP Binding Cassette Transporter A9 (ABCA9) and analogues was observed.
• Sequence Length: 1624

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A9 (ABCA9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A9 (ABCA9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A9 (ABCA9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A9 (ABCA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A9 (ABCA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter A9 (ABCA9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ABCA9 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 17223624
• NCBI GeneID: 10350
• Molecular Weight: 30,998 Da
• NCBI Official Full Name: ATP-binding cassette A9
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 9
• NCBI Official Symbol: ABCA9
• NCBI Official Synonym Symbols: EST640918
• NCBI Protein Information: ATP-binding cassette sub-family A member 9
• UniProt Protein Name: ATP-binding cassette sub-family A member 9
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA9
• UniProt Entry Name: ABCA9_HUMAN

NCBI Description

This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This gene is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of this gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA9: May play a role in monocyte differentiation and lipid homeostasis. Belongs to the ABC transporter superfamily. ABCA family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: mitochondrion; integral to membrane

Molecular Function: transporter activity; ATPase activity; ATP binding

Biological Process: metabolic process; transport