Western Blot (WB)
(KLHL1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the KLHL1 antibody detected the KLHL1 protein (arrow).)
Rabbit anti-Human, Mouse KLHL1 Polyclonal Antibody | anti-KLHL1 antibody
KLHL1, NT (KLHL1, KIAA1490, Kelch-like protein 1) (FITC)
Gene Names
KLHL1; MRP2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KLHL1; Polyclonal Antibody; NT (KLHL1; KIAA1490; Kelch-like protein 1) (FITC); Kelch-like protein 1; anti-KLHL1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-KLHL1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KLHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KLHL1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(KLHL1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the KLHL1 antibody detected the KLHL1 protein (arrow).)
Western Blot (WB)
(KLHL1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the KLHL1 antibody detected the KLHL1 protein (arrow).)
Related Product Information for anti-KLHL1 antibody
The KLHL1 protein belongs to a family of actin-organizing proteins related to Drosophila Kelch (Nemes et al., 2000 [PubMed 10888605]).
Product Categories/Family for anti-KLHL1 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Aromolaran, K.A., et al. Am. J. Physiol., Cell Physiol. 298 (6), C1353-C1362 (2010): Chen, W.L., et al. Brain Res. 1233, 176-184 (2008): Aromolaran, K.A., et al. Neuroscience 145(3):841-850(2007). Seng, S., et al. Mol. Cell. Biol. 26(22):8371-8384(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,680 Da
NCBI Official Full Name
kelch-like protein 1 isoform 1
NCBI Official Synonym Full Names
kelch-like family member 1
NCBI Official Symbol
KLHL1
NCBI Official Synonym Symbols
MRP2
NCBI Protein Information
kelch-like protein 1; Mayven-related protein 2
UniProt Protein Name
Kelch-like protein 1
Protein Family
UniProt Gene Name
KLHL1
UniProt Synonym Gene Names
KIAA1490
UniProt Entry Name
KLHL1_HUMAN
Uniprot Description
KLHL1: May play a role in organizing the actin cytoskeleton of the brain cells.
Chromosomal Location of Human Ortholog: 13q21
Cellular Component: cytoskeleton; cell soma; cytoplasm; dendrite
Molecular Function: actin binding
Biological Process: cerebellar Purkinje cell layer development; dendrite development; actin cytoskeleton organization and biogenesis; adult walking behavior
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Product Notes
The KLHL1 klhl1 (Catalog #AAA6314109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLHL1, NT (KLHL1, KIAA1490, Kelch-like protein 1) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KLHL1 klhl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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