Mouse Asymmetric dimethylarginine (ADMA) ELISA Kit | ADMA elisa kit

Mouse Asymmetric dimethylarginine (ADMA) ELISA Kit

Cat. Num. AAA263225

• Gene Names: AGXT2; AGT2; DAIBAT
• Reactivity: Mouse
• Synonyms: Asymmetric dimethylarginine (ADMA); Mouse Asymmetric dimethylarginine (ADMA) ELISA Kit; ADMA elisa kit

• Reactivity: Mouse
• Sequence Length: 514

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 5 umol/L-0.078umol/L
• Sensitivity: Up to 0.01 umol/L
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ADMA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse ADMA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 17432913
• NCBI GeneID: 64902
• Molecular Weight: 49,297 Da
• NCBI Official Full Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• NCBI Official Synonym Full Names: alanine--glyoxylate aminotransferase 2
• NCBI Official Symbol: AGXT2
• NCBI Official Synonym Symbols: AGT2; DAIBAT
• NCBI Protein Information: alanine--glyoxylate aminotransferase 2, mitochondrial; beta-ALAAT II; beta-alanine-pyruvate aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase
• UniProt Protein Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• UniProt Gene Name: AGXT2
• UniProt Synonym Gene Names: AGT2; AGT 2
• UniProt Entry Name: AGT2_HUMAN

NCBI Description

The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

Uniprot Description

AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

Protein type: Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.44; EC 2.6.1.40; Transferase

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding

Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; glyoxylate metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination