Mouse ASK1 ELISA Kit | ASK1 elisa kit

Mouse ASK1 (Apoptosis Signal Regulating Kinase 1) ELISA Kit

Cat. Num. AAA2509157

• Reactivity: Mouse
• Synonyms: ASK1; Mouse ASK1 (Apoptosis Signal Regulating Kinase 1) ELISA Kit; ASK1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ASK1. No significant cross-reactivity or interference between Mouse ASK1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ASK1 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 292

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASK1 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ASK1 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ASK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ASK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ASK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ASK1. You can calculate the concentration of Mouse ASK1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 269970311
• NCBI GeneID: 853814
• NCBI Accession #: NP_012872.2
• NCBI GenBank Nucleotide #: NM_001179618.1
• UniProt Accession #: P35734; Q68EC3; Q6B290; D6VXN5
• Molecular Weight: 32,072 Da
• NCBI Official Full Name: Ask1p
• NCBI Official Symbol: ASK1
• NCBI Protein Information: Ask1p
• UniProt Protein Name: DASH complex subunit ASK1
• Protein Family: DASH complex
• UniProt Gene Name: ASK1
• UniProt Entry Name: ASK1_YEAST

Uniprot Description

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.