Western Blot (WB)
(Western blot analysis of MBS609352: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).)
Mouse Estrogen Receptor, alpha Monoclonal Antibody | anti-ESR1 antibody
Estrogen Receptor, alpha (ERa)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot (ECL) (1): 2ug/ml
Gel Mobility Shift Assay (2): 20ug/reaction
Immunoprecipitation (2,3): 20ug/100ul beads. We recommend that protein A Sepharose be used as immunoabsorbent with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody.
Optimal dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of MBS609352: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).)
Western Blot (WB)
(Western blot analysis of MBS609352: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008]
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; steroid hormone receptor activity; chromatin binding; transcription factor activity; transcription factor binding; steroid binding
Biological Process: estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; negative regulation of transcription factor activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; gene expression; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription factor activity; steroid hormone mediated signaling
Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance
Research Articles on ESR1
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Product Notes
The ESR1 esr1 (Catalog #AAA609352) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Estrogen Receptor, alpha (ERa) reacts with Hamster, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor, alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot, Gel Mobility Shift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot (ECL) (1): 2ug/ml Gel Mobility Shift Assay (2): 20ug/reaction Immunoprecipitation (2,3): 20ug/100ul beads. We recommend that protein A Sepharose be used as immunoabsorbent with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor, alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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