Mouse Apelin (APLN) ELISA Kit | APLN elisa kit

Mouse Apelin (APLN) ELISA Kit

Cat. Num. AAA2021640

• Gene Names: APLN; APEL; XNPEP2
• Reactivity: Mouse
• Synonyms: Apelin (APLN); Mouse Apelin (APLN) ELISA Kit; XNPEP2; APEL; APJ endogenous ligand; APLN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apelin (APLN).; No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.
• Sequence Length: 77

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 98.77-8000pg/mL
• Sensitivity: < 35.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin (APLN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin (APLN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APLN elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin (APLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin (APLN) in the sample.

Product Categories/Family for APLN elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 45120090
• NCBI GeneID: 8862
• NCBI Accession #: NP_059109.3
• NCBI GenBank Nucleotide #: NM_017413.4
• UniProt Accession #: Q9ULZ1; Q4VY08; Q8WU89
• Molecular Weight: 8,569 Da
• NCBI Official Full Name: apelin preproprotein
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: APLN
• NCBI Official Synonym Symbols: APEL; XNPEP2
• NCBI Protein Information: apelin
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: APLN
• UniProt Synonym Gene Names: APEL
• UniProt Entry Name: APEL_HUMAN

NCBI Description

This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.[provided by RefSeq, Feb 2010]

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular space; extracellular region

Molecular Function: G-protein-coupled receptor binding; hormone activity; apelin receptor binding; receptor binding

Biological Process: lactation; immune response; signal transduction