Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-fetoprotein (AFP) Standard Curve.)
Mouse Alpha-Fetoprotein (AFP) ELISA Kit | AFP elisa kit
Mouse Alpha-Fetoprotein (AFP) ELISA Kit
Reactivity
Mouse
Synonyms
Alpha-Fetoprotein (AFP); Mouse Alpha-Fetoprotein (AFP) ELISA Kit; AFP; FETA; HPAFP; OTTHUMP00000160480; alpha-1-fetoprotein; alpha-fetoglobulin; AFP elisa kit
Reactivity
Mouse
Specificity
Mouse Alpha-fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse AFP. No significant cross-reactivity or interference between Mouse AFP and analogues was observed.
Sequence Length
605
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-fetoprotein (AFP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-fetoprotein (AFP) Standard Curve.)
Related Product Information for AFP elisa kit
Description: This Mouse Alpha-fetoprotein (AFP) ELISA Kit employs a two-site sandwich ELISA to quantitate AFP in samples. An antibody specific for AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate. During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life. AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer.
Background: AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate. During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life. AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha fetoprotein
NCBI Official Symbol
Afp
NCBI Protein Information
alpha-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
Protein Family
UniProt Gene Name
Afp
Uniprot Description
Binds estrogens, fatty acids and metals.
Research Articles on AFP
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Product Notes
The Mouse AFP afp (Catalog #AAA9717175) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717175 ELISA Kit recognizes Mouse AFP. It is sometimes possible for the material contained within the vial of "Alpha-Fetoprotein (AFP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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