Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Advanced glycation end products (AGEs) Standard Curve.)
Mouse Advanced Glycation End Products (AGEs) ELISA Kit | AGEs elisa kit
Mouse Advanced Glycation End Products (AGEs) ELISA Kit
Reactivity
Mouse
Synonyms
Advanced Glycation End Products (AGEs); Mouse Advanced Glycation End Products (AGEs) ELISA Kit; AGEs elisa kit
Reactivity
Mouse
Specificity
Mouse Advanced glycation end products (AGEs) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse AGEs. No significant cross-reactivity or interference between Mouse AGEs and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Advanced glycation end products (AGEs) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Advanced glycation end products (AGEs) Standard Curve.)
Related Product Information for AGEs elisa kit
Description: This Mouse Advanced glycation end products (AGEs) ELISA Kit employs a two-site sandwich ELISA to quantitate AGEs in samples. An antibody specific for AGEs has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGEs present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGEs is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGEs bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. Side products generated in intermediate steps may be oxidizing agents or not. "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation.' AGEs may be formed external to the body (exogenously) by heating (e.g., cooking) sugars with fats or proteins; or inside the body (endogenously) through normal metabolism and aging. Under certain pathologic conditions (e.g., oxidative stress due to hyperglycemia in patients with diabetes), AGE formation can be increased beyond normal levels. AGEs are now known to play a role as proinflammatory mediators in gestational diabetes as well.
Background: Advanced Glycation End products (AGEs) are the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. Side products generated in intermediate steps may be oxidizing agents or not. "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation.' AGEs may be formed external to the body (exogenously) by heating (e.g., cooking) sugars with fats or proteins; or inside the body (endogenously) through normal metabolism and aging. Under certain pathologic conditions (e.g., oxidative stress due to hyperglycemia in patients with diabetes), AGE formation can be increased beyond normal levels. AGEs are now known to play a role as proinflammatory mediators in gestational diabetes as well.
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Product Notes
The Mouse AGEs (Catalog #AAA9717177) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717177 ELISA Kit recognizes Mouse AGEs. It is sometimes possible for the material contained within the vial of "Advanced Glycation End Products (AGEs), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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