Mouse ADAM10 (Disintegrin and metalloproteinase domain-containing protein 10) ELISA Kit | ADAM10 elisa kit

Mouse ADAM10 (Disintegrin and metalloproteinase domain-containing protein 10) ELISA Kit

Cat. Num. AAA7607093

• Gene Names: ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• Reactivity: Mouse
• Synonyms: ADAM10 (Disintegrin and metalloproteinase domain-containing protein 10); Mouse ADAM10 (Disintegrin and metalloproteinase domain-containing protein 10) ELISA Kit; ADAM10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADAM10. No significant cross-reactivity or interference between ADAM10 and analogues was observed.
• Sequence Length: 748

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM10 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2393947
• NCBI GeneID: 102
• Molecular Weight: 49,047 Da
• NCBI Official Full Name: ADAM10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]

Uniprot Description

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146).