Typical Testing Data/Standard Curve
Mouse Activity-dependent neuroprotector homeobox protein (ADNP) ELISA Kit | ADNP elisa kit
Mouse Activity-dependent neuroprotector homeobox protein (ADNP) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay: ADNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADNP antibody and an ADNP-HRP conjugate. The assay sample and buffer are incubated together with ADNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADNP concentration since ADNP from samples and ADNP-HRP conjugate compete for the anti-ADNP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADNP from the sample, fewer sites are left to bind ADNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADNP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
ADNP: Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.
Protein type: DNA-binding; C2H2-type zinc finger protein; Apoptosis
Chromosomal Location of Human Ortholog: 20q13.13
Cellular Component: extracellular space; nucleus
Molecular Function: protein binding; DNA binding; metal ion binding; chromatin binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of neuron apoptosis
Disease: Mental Retardation, Autosomal Dominant 28
Research Articles on ADNP
Similar Products
Product Notes
The Mouse ADNP adnp (Catalog #AAA7230960) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230960 ELISA Kit recognizes Mouse ADNP. It is sometimes possible for the material contained within the vial of "Activity-dependent neuroprotector homeobox protein (ADNP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
