Mouse anti-Human YAP / YAP1 Monoclonal Antibody | anti-YAP antibody

Mouse Monoclonal [clone 2H1] (IgG2a,k) to Human YAP / YAP1

Cat. Num. AAA245656

• Gene Names: YAP1; YAP; YKI; COB1; YAP2; YAP65
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: YAP / YAP1; Monoclonal Antibody; Mouse Monoclonal [clone 2H1] (IgG2a; k) to Human YAP / YAP1; Anti-YAP / YAP1 Antibody (clone 2H1) IHC-plus; YAP1; 65 kDa Yes-associated protein; YAP; Yes-associated protein beta; Yes-associated protein delta; Yorkie homolog; YAP2; Yes-associated protein 1; Yes-associated protein 2; YKI; YAP65; Human YAP; anti-YAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2H1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-YAP antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (40 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control Hela NE. Immunofluorescence (40 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: YAP1 (NP_006097, 53 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: YAP / YAP1 antibody was raised against yAP1 (NP_006097, 53 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-YAP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-YAP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-YAP1 antibody IHC of human colon, muscularis mucosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 40 ug/ml).)

Western Blot (WB)

(YAP1 monoclonal antibody, clone 2H1 Western blot of YAP1 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 5174751
• NCBI GeneID: 10413
• NCBI Accession #: NP_006097.1
• NCBI GenBank Nucleotide #: NM_006106.3
• UniProt Accession #: P46937; Q7Z574; B4DTY1; B7ZA01; E3WEB5; E3WEB6; E9PRV2; F5H202; K0KQ18; K0KYZ8; K0L195; K0L1G3
• Molecular Weight: 54,942 Da
• NCBI Official Full Name: yorkie homolog isoform 2
• NCBI Official Synonym Full Names: Yes-associated protein 1
• NCBI Official Symbol: YAP1
• NCBI Official Synonym Symbols: YAP; YKI; COB1; YAP2; YAP65
• NCBI Protein Information: transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog
• UniProt Protein Name: Transcriptional coactivator YAP1
• UniProt Gene Name: YAP1
• UniProt Synonym Gene Names: YAP65; Yes-associated protein 1
• UniProt Entry Name: YAP1_HUMAN

NCBI Description

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Uniprot Description

YAP1: an adaptor protein that binds to HER4 and the SH3 domain of the Yes tyrosine kinase. Associates with multiple transcription factors in the nucleus, and appears to be a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Contains a WW domain that is found in various structural, regulatory and signaling molecules.

Protein type: Transcription, coactivator/corepressor; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; membrane; cytoplasm; cell junction; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription corepressor activity

Biological Process: paraxial mesoderm development; transcription initiation from RNA polymerase II promoter; somatic stem cell maintenance; lateral mesoderm development; cell morphogenesis; negative regulation of epithelial cell differentiation; notochord development; positive regulation of organ growth; keratinocyte differentiation; cell proliferation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; gene expression; vasculogenesis; response to DNA damage stimulus

Disease: Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/palate, And/or Mental Retardation