Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Actin, cytoplasmic 1 (ACTB) Standard Curve.)
Mouse Actin, Cytoplasmic 1 (ACTB) ELISA Kit | ACTB elisa kit
Mouse Actin, Cytoplasmic 1 (ACTB) ELISA Kit
Gene Names
Actb; Actx; beta-actin; E430023M04Rik
Reactivity
Mouse
Synonyms
Actin; Cytoplasmic 1 (ACTB); Mouse Actin; Cytoplasmic 1 (ACTB) ELISA Kit; ACTB; PS1TP5BP1; PS1TP5-binding protein 1; actin; cytoplasmic 1; beta actin; beta cytoskeletal actin; ACTB elisa kit
Reactivity
Mouse
Specificity
Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACTB. No significant cross-reactivity or interference between Mouse ACTB and analogues was observed.
Sequence Length
375
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Actin, cytoplasmic 1 (ACTB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Actin, cytoplasmic 1 (ACTB) Standard Curve.)
Related Product Information for ACTB elisa kit
Description: This Mouse Actin, cytoplasmic 1 (ACTB) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTB in samples. An antibody specific for ACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Beta-actin (gene name ACTB) is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. In a neoplastic cell line resulting from treatment of cultured human diploid fibroblasts with a chemical mutagen, Leavitt et al. (1982) observed a mutant form of beta actin. Toyama and Toyama (1984) isolated and characterized lines of KB cells resistant to cytochalasin B. They found that one resistant line had an alteration in beta-actin. Such cells bound less cytochalasin B than did parental KB cells. The authors suggested that the primary site of action of cytochalasin B on cell motility processes is beta-actin.
Background: Beta-actin (gene name ACTB) is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. In a neoplastic cell line resulting from treatment of cultured human diploid fibroblasts with a chemical mutagen, Leavitt et al. (1982) observed a mutant form of beta actin. Toyama and Toyama (1984) isolated and characterized lines of KB cells resistant to cytochalasin B. They found that one resistant line had an alteration in beta-actin. Such cells bound less cytochalasin B than did parental KB cells. The authors suggested that the primary site of action of cytochalasin B on cell motility processes is beta-actin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
Actb
NCBI Official Synonym Symbols
Actx; beta-actin; E430023M04Rik
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
Protein Family
UniProt Gene Name
Actb
NCBI Description
This gene encodes a member of the actin family of proteins. Actins are highly conserved proteins that are among the most abundant proteins in eukaryotic cells and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the transcribed mRNA are regulated through the binding of multiple factors to its 3' UTR sequence. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous pseudogenes of this gene have been identified in the mouse genome. [provided by RefSeq, Sep 2015]
Uniprot Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Articles on ACTB
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Product Notes
The Mouse ACTB actb (Catalog #AAA9717152) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717152 ELISA Kit recognizes Mouse ACTB. It is sometimes possible for the material contained within the vial of "Actin, Cytoplasmic 1 (ACTB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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