Mouse 17-Hydroxypregnenolone ELISA Kit
Mouse 17-Hydroxypregnenolone ELISA Kit
Reactivity
Mouse
Synonyms
17-Hydroxypregnenolone; Mouse 17-Hydroxypregnenolone ELISA Kit; 17-Hydroxypregnenolone elisa kit
Reactivity
Mouse
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 nmol/L.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 17-Hydroxypregnenolone elisa kit
Intended Uses: This 15S-HETE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 15S-HETE. This ELISA kit for research use only!
Principle of the Assay: 15S-HETE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-15S-HETE antibody and an 15S-HETE-HRP conjugate. The assay sample and buffer are incubated together with 15S-HETE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 15S-HETE concentration since 15S-HETE from samples and 15S-HETE-HRP conjugate compete for the anti-15S-HETE antibody binding site. Since the number of sites is limited, as more sites are occupied by 15S-HETE from the sample, fewer sites are left to bind 15S-HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 15S-HETE concentration in each sample is interpolated from this standard curve.
Principle of the Assay: 15S-HETE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-15S-HETE antibody and an 15S-HETE-HRP conjugate. The assay sample and buffer are incubated together with 15S-HETE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 15S-HETE concentration since 15S-HETE from samples and 15S-HETE-HRP conjugate compete for the anti-15S-HETE antibody binding site. Since the number of sites is limited, as more sites are occupied by 15S-HETE from the sample, fewer sites are left to bind 15S-HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 15S-HETE concentration in each sample is interpolated from this standard curve.
Product Categories/Family for 17-Hydroxypregnenolone elisa kit
