Typical Testing Data/Standard Curve (for reference only)
Mouse 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3) ELISA Kit | AGPAT3 elisa kit
Mouse 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AGPAT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AGPAT3 antibody and an AGPAT3-HRP conjugate. The assay sample and buffer are incubated together with AGPAT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGPAT3 concentration since AGPAT3 from samples and AGPAT3-HRP conjugate compete for the anti-AGPAT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGPAT3 from the sample, fewer sites are left to bind AGPAT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGPAT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
AGPAT3: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Acetyltransferase; Lipid Metabolism - glycerolipid; EC 2.3.1.51; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Membrane protein, integral
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; plasma membrane; nuclear envelope
Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process
Research Articles on AGPAT3
Similar Products
Product Notes
The Mouse AGPAT3 agpat3 (Catalog #AAA7200885) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200885 ELISA Kit recognizes Mouse AGPAT3. It is sometimes possible for the material contained within the vial of "1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
