CD3/CD8 Dual Color Reagent | CD3 dual color reagent

MOUSE ANTI HUMAN CD3:FITC/CD8:RPE

Cat. Num. AAA239273

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3/CD8; MOUSE ANTI HUMAN CD3:FITC/CD8:RPE; CD3 dual color reagent

• Host: Mouse
• Isotype: FITC reagent: IgG1 (MOUSE) RPE Reagent: IgG1 (MOUSE)
• Clone Number: UCHT1/LT8
• Specificity: UCHT1 recognises the human CD3 antigen, which is expressed by all mature T cells.LT8 recognises the human CD8 antigen, a 32kD dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells. ; ; This item may be used to simultaneously identify CD3 and CD8 positive cells and to identify populations of lymphocytes that co-express these antigens.
• Form/Format: FITC/RPE; Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
• Sequence Length: 235

• Applicable Applications for CD3 dual color reagent: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 0.5 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. This product should be stored undiluted. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(MBS239273 staining of human lymphocytes)

Related Product Information for CD3 dual color reagent

UCHT1 recognises the human CD3 antigen, which is expressed by all mature T cells. LT8 recognises the human CD8 antigen, a 32kD dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells. MBS239273 may be used to simultaneously identify CD3 and CD8 positive cells and to identify populations of lymphocytes that co-express these antigens.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345.1
• NCBI GenBank Nucleotide #: NM_001145873.1
• Molecular Weight: 30,214 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding

Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; cytotoxic T cell differentiation; positive regulation of calcium-mediated signaling; immune response; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Cd8 Deficiency, Familial