Mouse Inhibin A (INHA) DIY ELISA Kit | INHA diy elisa kit

Mouse Inhibin A (INHA) ELISA Kit DIY Materials

Cat. Num. AAA2088545

• Reactivity: Mouse
• Synonyms: Inhibin A (INHA); Mouse Inhibin A (INHA) ELISA Kit DIY Materials; INH-A; INHA diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Inhibin A (INHA). No significant cross-reactivity or interference between Inhibin A (INHA) and analogues was observed.
• Sequence Length: 366

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 12.35-1,000pg/mL
• Sensitivity: 4.67pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for INHA diy elisa kit

Endocrinology; Reproductive science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4504697
• NCBI GeneID: 3623
• NCBI Accession #: NP_002182.1
• NCBI GenBank Nucleotide #: NM_002191.3
• UniProt Accession #: P05111; A8K8H5
• Molecular Weight: 39,670 Da
• NCBI Official Full Name: inhibin alpha chain preproprotein
• NCBI Official Synonym Full Names: inhibin alpha subunit
• NCBI Official Symbol: INHA
• NCBI Protein Information: inhibin alpha chain
• UniProt Protein Name: Inhibin alpha chain
• Protein Family: Inhibin
• UniProt Gene Name: INHA

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients. [provided by RefSeq, Aug 2016]

Uniprot Description

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular region

Molecular Function: cytokine activity; growth factor activity; hormone activity; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell cycle arrest; cell development; cell differentiation; cell surface receptor linked signal transduction; cell-cell signaling; hemoglobin biosynthetic process; negative regulation of B cell differentiation; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; negative regulation of macrophage differentiation; negative regulation of phosphorylation; positive regulation of follicle-stimulating hormone secretion; regulation of apoptosis; regulation of cell cycle; regulation of cell proliferation; regulation of MAPKKK cascade; response to external stimulus; signal transduction; skeletal development