Mouse Dickkopf Related Protein 3 (DKK3) DIY ELISA Kit | DKK3 diy elisa kit

Mouse Dickkopf Related Protein 3 (DKK3) ELISA Kit DIY Materials

Cat. Num. AAA2089342

• Gene Names: DKK3; RIG; REIC
• Reactivity: Mouse
• Synonyms: Dickkopf Related Protein 3 (DKK3); Mouse Dickkopf Related Protein 3 (DKK3) ELISA Kit DIY Materials; REIC; DKK3 diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.
• Sequence Length: 350

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for DKK3 diy elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 66346689
• NCBI GeneID: 27122
• NCBI Accession #: NP_001018067.1
• NCBI GenBank Nucleotide #: NM_001018057.1
• UniProt Accession #: Q9UBP4; Q9ULB7; A8K1I2; D3DQW1
• Molecular Weight: 38,390 Da
• NCBI Official Full Name: dickkopf-related protein 3 isoform 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 3
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3
• UniProt Protein Name: Dickkopf-related protein 3
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Synonym Gene Names: REIC; Dickkopf-3; Dkk-3; hDkk-3

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.3

Biological Process: adrenal gland development; anatomical structure morphogenesis; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent