Human Lymphotoxin Beta ELISA Kit | LTbeta elisa kit

Human Lymphotoxin Beta ELISA Kit

Cat. Num. AAA760960

• Gene Names: LTB; p33; TNFC; TNFSF3; TNLG1C
• Reactivity: Human
• Synonyms: Lymphotoxin Beta; Human Lymphotoxin Beta ELISA Kit; LTb ; LTbeta elisa kit

• Reactivity: Human
• Sequence Length: 244

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LTbeta elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LTbeta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LTbeta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LTbeta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LTbeta can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4337113
• NCBI GeneID: 4050
• Molecular Weight: 7,780 Da
• NCBI Official Full Name: lymphotoxin beta
• NCBI Official Synonym Full Names: lymphotoxin beta
• NCBI Official Symbol: LTB
• NCBI Official Synonym Symbols: p33; TNFC; TNFSF3; TNLG1C
• NCBI Protein Information: lymphotoxin-beta
• UniProt Protein Name: Lymphotoxin-beta
• UniProt Gene Name: LTB
• UniProt Synonym Gene Names: TNFC; TNFSF3; LT-beta; TNF-C
• UniProt Entry Name: TNFC_HUMAN

NCBI Description

Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LTB: Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to membrane; plasma membrane

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding

Biological Process: cell-cell signaling; gene expression; immune response; lymph node development; positive regulation of interleukin-12 biosynthetic process; signal transduction; skin development; tumor necrosis factor-mediated signaling pathway