Mouse Inhibin A (INHA) CLIA Kit | INHA clia kit

Mouse Inhibin A (INHA) CLIA Kit

Cat. Num. AAA2707890

• Gene Names: Inha; AW555078
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Inhibin A (INHA); Mouse Inhibin A (INHA) CLIA Kit; Inhibin A; INH-A; INHA clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Inhibin A (INHA).; No significant cross-reactivity or interference between Inhibin A (INHA) and analogues was observed.
• Sequence Length: 366

• Applicable Applications for INHA clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 12.35-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.11pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin A (INHA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin A (INHA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Inhibin A (INHA). A competitive inhibition reaction is launched between biotin labeled Inhibin A (INHA) and unlabeled Inhibin A (INHA) (Standards or samples) with the pre-coated antibody specific to Inhibin A (INHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin A (INHA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Inhibin A (INHA) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 40254367
• NCBI GeneID: 16322
• NCBI Accession #: NP_034694.3
• NCBI GenBank Nucleotide #: NP_034694.3
• UniProt Accession #: Q04997
• NCBI Official Full Name: inhibin alpha chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: inhibin alpha
• NCBI Official Symbol: Inha
• NCBI Official Synonym Symbols: AW555078
• NCBI Protein Information: inhibin alpha chain
• UniProt Protein Name: Inhibin alpha chain
• Protein Family: Inhibin
• UniProt Gene Name: Inha

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mice in which this gene has been ablated exhibit sex cord-stromal tumors and symptoms that mimic the human cancer cachexia syndrome. [provided by RefSeq, Aug 2016]

Uniprot Description

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Inhibin deficient mice are viable but are acutely sensitive to development of gonadal sex-cord stromal tumors.